The game features the Teen Titans (Robin, Raven, Cyborg, Starfire, and Beast Boy) as playable characters in story mode. Players are able to switch between any of the five Titans in real time, each with unique fighting abilities, and the game allows up to four players simultaneously.

Aside from the game's story mode, players can fight against each other in a versus battle mode, known as Master of Games, with 31 unlockable characters.

The Teen Titans receive a video game in the mail, one that is starring them. When Cyborg and Beast Boy try to play it, they all get placed inside of the video game's world. They go through various levels as they try to figure out how to return home, fighting against several enemies and villains they have faced off against before.

After the Titans defeat most of the villains, Slade appears before them, making Robin suspect that he was behind everything, but Slade, too, is part of the program. The Master of Games then reveals himself as the mastermind, but after the Titans capture him, they find that he is not the true culprit. Breaking the fourth wall, the Titans reveal that the player is behind everything.